Regulatory

Part:BBa_K5159010

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-09-26)


Yarrowia lipolytica pLEU2

The following sequence activates the transcription of LEU2 gene in Yarrowia lipolytica, and is placed in front of the LEU2 coding sequence in the LEU2 marker expression cassette to drive its expression for auxotrophic selection.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal NgoMIV site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None